Contact an Account ManagerArachidonic acid metabolites from polymorphonuclear leukocytes of healthy donors severely burned patients and children with cystic fibrosis
Summary
Type: HPLC
Application #: 100490
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: UV 235nm and 280nm, wavelength switch at 38min
Conditions: eluent A: phosphate buffer - acetonitrile - methanol (50:30:20),
eluent B: phosphate buffer - acetonitrile - methanol (28:42:30),
solvent change at 34min,
flow rate 1ml/min,
column temperature 40 °C
Authors: Knöller J
Source: J Chromatogr 427 (1988) 199 - 208
Details
Legend: HPLC analysis of a cell supernatant [PMNLs (1.107) from a healthy donor
stimulated with Ca-ionophore (7.3µM) for 5min]. Time for analysis 70min.
3. LTC4,
6. 6-trans-LTB4,
7. 12-epi,6-trans-LTB4,